Guides
Practical guides for lab data.
Short, referenced explanations of the analyses researchers ask about most.
ELISA
How to analyse ELISA data: 4PL, 5PL and weighting
Turn plate-reader absorbances into reliable concentrations: blank correction, the right curve model, weighting and quality checks.
StatisticsWhich statistical test should I use?
A practical map from the shape of your experiment to the test, with lab examples.
ReportingARRIVE 2.0 checklist explained
What the ARRIVE 2.0 guidelines ask for when you report animal research, and how to prepare as you go.
Molecular biologyqPCR analysis with the ΔΔCt method
From Ct values to fold change, and which numbers to use for statistics.
MetabolismGlucose tolerance tests: curves and area under the curve
How to run, analyse and report a glucose tolerance test in mice and other models.
Study designHow to choose a sample size for an animal study
Enough animals to answer the question, and no more: the five numbers you need.
Step-by-step help with the program: Tutorials.