Free online tools

ELISA curve fitting and sample size, free in your browser.

Nothing is uploaded: the calculations run on your computer. The full PreclinIQ program adds plate import, five more curve models, statistics, figures and reports.

ELISA standard curve (4PL / 5PL)

Standards: one per line, concentration then one or more OD readings (tab, comma or space separated). Samples: ID, OD, dilution factor.

StandardsSamples

Blank ODModelWeightingUnit

Sample size for two groups

How many animals or participants per group to detect a difference between two means.

I know Control meanExpected change (%) Standard deviationSignificance (α)Power (%)TailsExpected loss (%)

Results are for planning and teaching. Check important calculations with a statistician. Read: How to analyse ELISA data · How to choose a sample size.